Modern Improvements in HPLC Column Technology

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Dorothy J. Phillips;Uwe D. Neue;Tom Walter, Kevin Wyndham;John O''Gara [Waters]
Fall ACS 2002; 224th ACS National Meeting; Boston August 18-22
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Atlantis dC18 5 µm Steel 4.6 mm 150 mm SymmetryShield(™) RP8 5 µm Steel 3.9 mm 150 mm Symmetry® C18 5 µm Steel 3.9 mm 150 mm XTerra MS C18 5 µm Steel 4.6 mm 150 mm
The analytical challenges of the pharmaceutical industry continue to drive the development of HPLC column technology. The demands for selectivity, speed and sensitivity became difficult to meet with the traditional silica-based columns. Over the last few years continued improvements have steadily been made in the technology of HPLC columns to meet these challenges. In this lecture, we will review some of the progress together with an outlook on the immediate future. We will review the history of high-purity silica, improved surface modification through stationary phases with embedded polar groups, and the development of pH stable organic-inorganic hybrid packings. Part of the discussion will also be the impact of these technologies on the ease with which new HPLC methods can be developed in the analytical laboratory. For example, the new hybrid technology expands the operating pH range for HPLC, thus facilitating method development.

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