Development of a New HPLC Column for the Retention and Separation of Highly Polar Compounds

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Diane Wagrowski-Diehl;Eric Grumbach;KimVan Tran;Christina Carignan;Pamela Iraneta [Waters]
PittCon 2002; New Orleans; 18 March 2002
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Other Symposium
--Non-polar compounds: Uracil; Triamcinolone; Hydrocortisone; 2-Amino-7-chloro-5-oxo-5H-[1]-benzopyrano[2,3-b]pyridinecarbonitrile; 6alpha-Methyl-17alpha-hydroxyprogesterone; 3-Aminofluoranthene; 2-Bromofluorene; Perylene; Naphthol[2,3-a]pyrene; --Polar compounds: Thiourea; 5-Fluorocytosine; Adenine; Guanosine-5''-monophosphate; Thymine; --Catecholamines: Noradrenaline; Adrenaline; 3,4-Dihydroxyphenylalanine; Dopamine; 3-Methoxy-4-hydroxyphenylglycol; 4-Hydroxy-3-methoxymandelic acid; Serotonin; 3,4-Dihydroxyphenylacetic acid; 5-Hydroxy-3-indoleacetic acid; Homovanillic acid; --Nucleic Acids: Cytosine; 5-Fluorouracil; Guanine; --Peptides: L2275; A6677; Bradykinin; Angiotensin I; Angiotensin II; Substance P; Renin Substrate; Insulin B Chain; Melittin
Atlantis dC18 5 µm Steel 4.6 mm 150 mm
Achieving useful retention of polar compounds on traditional C18 bonded phases is difficult. These phases suffer from poor performance using mobile phases with >90% water. We describe a new phase that affords much better performance for polar compounds. This phase is a medium coverage C18 that performs very well for the retention of polar neutrals and bases. Moreover, it can be used at very low pH to enhance retention of polar acids. The phase is also compatible with 100% aqueous mobile phases. Several applications on this new phase will be demonstrated.

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