Improving Our Understanding of Reversed-Phase Separations for the 21st Century

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Patrick D. McDonald, Bonnie A. Alden, KimVan Tran, Charles H. Phoebe, Jr., Pamela C. Iraneta, Mark Capparella, Thomas H. Walter, Uwe D. Neue, Barbara K. Grover, John E. O''Gara, Joseph C. Arsenault, Yuehong Xu, Pamela A. Richards [Waters]
Lecture, ISC 2000, London, 3 October 2000
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See also WA10200 and WA39000 in this database.

Abstract: Experiments designed to explore the actual physical reality of the interaction of mobile and stationary phases are fraught with practical limitations that impede drawing significant conclusions from the results. It has been easier for most practitioners to foster the folklore forged through the citation generations of HPLC literature than to refine the "cartoon-level" view of the chromatographic process to a higher "art".

Hybrid Particle Technology enables creation of chromatographic substrates with interesting, specifically designed chemical modifications that reside not simply at the "accessible" surface, but, rather, throughout the entire backbone of a particle''s molecular make-up. This now permits rational exploration of structure-activity relationships with a view toward understanding and explaining observations that previously seemed incongruous. Accessible-surface modifications in the traditional manner add further variables to the study of the ways in which analyte molecules interact with the mobile and stationary phases on their "random walk" through the chromatographic bed.

Results of experiments using both bonded-silica phases and new bonded-hybrid particle phases, allied with recent ideas from related disciplines, will be shown to challenge traditional concepts of accessible surface, ligand density, silanol interaction, and hydrophobic "collapse". Particular attention will be paid to the role of the structure and physicochemical properties of both particle substrate and mobile phase elements as they, together, determine the constitution and function of the "stationary phase".

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