Analysis of Aminoglycosides in Foods Using a Zwitterionic Stationary Phase and Liquid Chromatography-Tandem Mass Spectrometry

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Jinchuan Yang, Paul D. Rainville
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Application Notes
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Application Notes
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This application note demonstrates about HILIC-MS/MS method which provides a good solution for the screening and quantification of AMGs in foods. The effects of chromatographic conditions, including mobile phase composition, buffer concentration, and pH, on the separation of AMGs on the Atlantis Premier BEH Z-HILIC Column have been systematically investigated. Unlike other zwitterionic sulfoalkylbetaine stationary phases with which high concentrations of ammonium formate up to 175 mM are required, the Atlantis Premier BEH Z-HILIC Column achieves adequate separation and excellent sensitivity for 17 common AMGs in a gradient elution with aqueous 20 mM ammonium formate at pH 3.0 and acetonitrile with 0.1% FA in a 10 minute run. The Oasis HLB SPE Cartridge provided satisfactory cleanup and recoveries for 16 AMGs.

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