Automated RapiFluor-MS Labeled Glycan Sample Preparation for Disulfide-Rich Glycoprotein

Library Number:
APNT135090582
Part Number:
720007402EN
Author(s):
Yamin Htet, Stephan M. Koza
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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This application note demonstrates N-glycans are routinely monitored for the quality of biotherapeutics because they affect the safety and efficacy of many therapeutic proteins. Current glycan analysis is developed with the well-studied glycans from monoclonal antibodies (mAb). Here, we present a complementary method that provides a reproducible profile of N-glycans released from Human Chorionic Gonadotrophin (hCG), a highly complex glycoprotein with multiple disulfide bonds. The automated reduced protein protocol is suitable for commercial as well as research environments.


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