Methods for the Anion Exchange Chromatographic Analysis of mRNAs

Library Number:
APNT135089970
Part Number:
720007412EN
Author(s):
Szabolcs Fekete, Hua Yang, Stephan M. Koza, Kevin Wyndham, Matthew A. Lauber
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 

In this application note, we demonstrate that using an anion exchange column between 30–50 °C at neutral pH might be suitable for this type of analysis. Both ion pairing and classical salt gradient conditions can be applied. The effect of temperature on the anion exchange column is discussed and how it might be utilized to detect conformational changes to the mRNA molecules.


Title Format File Size
Download PDF PDF 228.6kB