A Complete Solution for Targeted Lipid Analysis Using a Smart Compact LC-Tof

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Giorgis Issac, Lisa Reid, Lee Gethings, Jayne Kirk
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Routine lipid analysis of blood and tissue samples in biomedical research requires a robust, rapid and simple analysis of identified lipids of interest. Once a potential biological marker of disease has been hypothesised and identified a focused analysis for specific lipids of interest, or class changes, can be performed to test the validity of the biomedical hypothesis. This can be achieved using simple sample preparation, non‑expert instrument usage and easy to use semi-automated data processing. 

To demonstrate: cancer vs healthy human plasma samples, NIST plasma and Avanti SPLASH lipidomic standards were protein precipitated, separated using reversed‑phase UPLC and analysed in ESI+ on the ACQUITY RDa Detector. Data were acquired and processed using UNIFI software with targets identified against a curated lipid library. The workflow generated sensitive, accurate and robust data, and was processed simply and rapidly with the commercially available peak identification and data comparison software packages.

The robustness, mass accuracy, dynamic range and limit of detection (LOD) of the workflow was assessed with a continuous acquisition totaling >6 days. Over the entire analysis this workflow demonstrated excellent chromatographic retention consistency, with peaks of interest varying by a maximum of 0.81%. The signal intensity and peak area showed a <10% deviance in both signal count and calculated analyte response, the mass accuracy for the 9 standards averaged ±6.5 ppm. Data comparison processing simplicity and efficacy was demonstrated using plasma taken from healthy donors and cancer patients.

This workflow: delivered by the ACQUITY RDa MS platform, has great potential to fulfill a range of basic lipid screening requirements. Combining the complete instrument set-up, acquisition and processing package allows for greater efficiencies and a more automated approach, improving the accessibility of mass spectrometry analysis, thus enabling a wider range of scientists and organisations to utilize these techniques.

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