Analysis of Phosphatidylethanol (PEth) in Whole Blood using SPE and UPLC-MS/MS for Forensic Toxicology

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Jonathan P. Danaceau, Michelle Wood
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We have developed a bioanalytical method for the quantitative analysis of PEth 16:0/18:1 and PEth 16:0/18:2 in whole blood. The method uses protein precipitation followed by a simple extraction using Ostro Pass-Through Sample Preparation plates. Analysis using the Waters ACQUITY UPLC System and Xevo TQ-S micro Mass Spectrometer resulted in a method that was linear, accurate and precise, had no detectable carryover, and had the analytical sensitivity necessary to easily meet the suggested cutoff levels of 0.05 µM.

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