Repeatability of the ACQUITY UPLC H-Class PLUS Bio Binary System Under Challenging Separation Conditions

Library Number:
APNT135078149
Part Number:
720007086EN
Author(s):
David Dao, Brooke M. Koshel, Robert E. Birdsall, Ying Qing Yu
Source:
Waters Corporation
Content Type:
Application Notes
Content Subtype:
Application Notes
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Peptide mapping analyses often employ low flow rates and shallow gradients, conditions that are considered challenging for developing robust analytical methods. Although some variability in retention time is expected under these conditions, retention time repeatability is critical for proper peptide identification and quantitation. This work demonstrates that the ACQUITY UPLC H-Class PLUS Bio Binary System can be used to deliver exceptional gradient precision and to minimize the retention time variability experienced under challenging method conditions.


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