Analysis of Cysteine-Conjugated Antibody Drug Conjugates (ADCs) Using a Native SEC LC-MS Workflow on the SYNAPT XS

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Henry Shion, Colette Quinn, Scott J. Berger, Ying Qing Yu
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Application Notes
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In this application brief, we have demonstrated an analytical scale native SEC LC-MS analysis workflow that can be applied across LC-MS systems for the analysis of cysteine conjugated ADCs. SEC-MS based workflows offer the efficiency benefits of automated sample operation over infusion-based analysis and removes the need for sample preparation (e.g. buffer exchange and deglycosylation) prior to MS analysis of DAR and drug distribution analysis for ADC molecules.

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