Analysis of Cysteine-Conjugated Antibody Drug Conjugates (ADCs) Using a Native SEC LC-MS Workflow on the SYNAPT XS

Library Number:
APNT135075751
Part Number:
720007026EN
Author(s):
Henry Shion, Colette Quinn, Scott J. Berger, Ying Qing Yu
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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In this application brief, we have demonstrated an analytical scale native SEC LC-MS analysis workflow that can be applied across LC-MS systems for the analysis of cysteine conjugated ADCs. SEC-MS based workflows offer the efficiency benefits of automated sample operation over infusion-based analysis and removes the need for sample preparation (e.g. buffer exchange and deglycosylation) prior to MS analysis of DAR and drug distribution analysis for ADC molecules.


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