Improved Oligonucleotide SPE-LC-MS Analysis Using MaxPeak High Performance Technology

Library Number:
APNT135075015
Part Number:
720007019EN
Author(s):
Kathryn Brennan, Mary Trudeau Lame, Michael Donegan, Paul D. Rainville
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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The demand for highly selective and sensitive LC-MS bioanalytical assays in support of research and development of next-generation oligonucleotide therapies has greatly increased. The work described herein uses SPE, RP-UPLC with the sub-2-μm ACQUITY PREMIER Oligonucleotide C18 Column, and tandem-quadrupole MS for detection and quantification of oligodeoxythymidines and the fully phosphorothioated oligonucleotide antisense therapy, GEM91.


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