Automated Medium and High-Throughput GlycoWorks RapiFluor-MS Preparations on the Andrew+ Pipetting Robot

Library Number:
APNT135074361
Part Number:
720007008EN
Author(s):
Corey E. Reed, Stephan M. Koza, Steven Calciano
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Technical Notes
Related Products:
 
 
 
 
 

Using the Andrew+ pipetting robot from Andrew Alliance, the GlycoWorks RapiFluor-MS N-glycan Labeling 24 and 48 Sample Kits were automated and verified for performance. Verification requirements included comparability to manually performed preparations of the same sample number with ≤25% deviation in total area and ≤5% deviation in relative area. Additionally, intra-preparation relative standard deviations must meet these same requirements. Ultimately, the 24-sample automated protocol showed 12.3% variation from manual preparations in terms of total area and 0.0% deviation in terms of relative area. The automated 48-sample protocol was also comparable to the 24-sample manual preparation with a 23.0% deviation in terms of total area and 0.0% deviation in terms of relative area. The automated 48-sample protocol also showed excellent intra-preparation reproducibility with 11.6% and 15.2% total area relative standard deviation over the 2 preparations.


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