Differentiation of Natural Vitamin E from Synthetic Vitamin E for Food and Dietary Supplement Nutrition Labeling

Library Number:
APNT135072984
Part Number:
720006924EN
Author(s):
Jinchuan Yang Paul D. Rainville
Source:
Waters Corporation
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
Food QC & Nutritional Testing
 
 

Waters ACQUITY UPC2 System with Trefoil AMY1 and CEL1 Columns provide a simple and fast solution for differentiation between the natural vitamin E and the synthetic vitamin E. The stereoisomers of α-tocopherol and its acetate can be separated into 2 or more peaks. Based on the chromatographic profile, natural vitamin E can be easily differentiated from synthetic vitamin E. The sample preparation for these analyses is simple. There is no derivatization required. The chromatographic analysis run time is 35 min for the α-tocopherols, and 15 min for the α-tocopheryl acetate, respectively. For the first time, the differentiation between natural and synthetic vitamin E can be easily conducted. These analytical methods are suitable for the routine analysis of vitamin E in foods, dietary supplements, and other related products. 


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