A Robust and Reproducible Reversed-Phase Lipid Profiling Method for Large Sample Sets

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Giorgis Isaac, Nyasha Munjoma, Lee A. Gethings, Lauren Mullin, Robert S. Plumb
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A robust and reproducible high resolution UPLC-MS method has been developed for the detection and identification of lipid species in plasma, with average retention time percent relative standard deviation of less than 0.19 across the analysis (n=100). Despite the short analysis time, 10 minutes, the method produced a peak capacity of 90, allowing for the excellent separation of the various lipid classes in the NIST human plasma sample. The developed method is readily applicable to a broad range of biological samples such as body fluids, tissue samples, and cell cultures.


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