MetaboQuan-R for the UPLC-MS/MS Analysis of Steroids

Library Number:
APNT135071748
Part Number:
720006936EN
Author(s):
Billy J. Molloy, Robert Wardle, Dominic Foley, Robert S. Plumb
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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A rapid UPLC-MS/MS methodology has been developed for the research analysis of 14 endogenous steroids in human plasma and serum. This method demonstrated to be suitable for the analysis of these steroids at physiologically relevant levels in human plasma and serum. This method utilizes a flexible reversed-phase UPLC-MS/MS platform that can be used for various compound classes (including bile acids, free fatty acids, amino acids, tryptic peptides, acyl-carnitines, and lipids); thus allowing the assay to be employed as part of a suite of methodologies that can be operated sequentially as part of a targeted multi-omics workflow with broad compound coverage.

 


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