Comprehending COVID-19: Reversed-Phase Liquid Chromatography (RPLC) of Intact SARS-CoV-2 Spike Protein

Library Number:
APNT135071700
Part Number:
720006907EN
Author(s):
Jennifer M. Nguyen, Matthew A. Lauber
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Technical Notes
Related Products:
 
Biopharmaceutical Discovery
 
 
 

The global COVID-19 pandemic has resulted in extensive efforts to develop vaccines for the novel coronavirus. Identifying vaccine targets relies on robust analytical methods to understand SARS-CoV-2 structural biology. This study focuses on reversed-phase liquid chromatographic analysis of the intact SARS-CoV-2 spike protein, which has emerged as a potential target for vaccine development due to its role in viral pathogenesis. This work demonstrates that using difluoroacetic acid (DFA) as a mobile phase modifier in place of formic acid (FA) results in increased chromatographic resolution during intact protein analysis. Furthermore, the results suggest that pairing this approach with N- and O-glycosidase treatments may enable more detailed intact protein MS investigations.


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