Size-Exclusion Chromatography (SEC) of Proteins and Peptides Application Notebook

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Gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic technique that separates compounds based on their relative different size in solution, which loosely correlates with their molecular weight. For over 50 years, Waters™ has been the market leaderin GPC- and SEC-based analyses, providing the highest quality columns , instrumentation, and applications support for both organic- and aqueous-based size separations.

SEC is the analytical "gold standard" for the separation and accurate quantitation of aggregates, monomers, and lower molecular weight fragments contained in biotherapeutic peptides and proteins, including monoclonal antibody (mAbs) drugs. There are more than 500 mAbs usedas biotherapeutics, diagnostic reagents, or research tools, many of which require the use of SEC to help confirm their quality for the intended end use. 

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