Application of Intentionally Attenuated MRM Transitions for the Analysis of Natural Products

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Douglas Stevens, Kari Organtini, Marian Twohig, Peter Hancock
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The combined use of the 13C isotope peak for the precursor and lower abundance, higher specificity fragments for intentionally attenuated MRM transitions demonstrated the ability to reduce the response of high abundance compounds, cannabinoids in cannabis and hemp, in order to have their relative response made comparable to other low abundance components, terpenoids, of the homogenized biomass extracted for this study. This makes quantitation of compound classes of disparate relative abundance more readily compatible with the EI GC-MS/MS technique often applied to the analysis of natural products. 

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