Utilizing UPLC Separation to Enable the Analysis of 19 Steroid Hormones in Serum for Clinical Research

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Robert Wardle, Dominic Foley, and Lisa Calton
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Application Notes
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Technical Notes
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A selective UPLC-MS/MS clinical research method has successfully been developed for the analysis of 19 serum steroid hormones. Chromatographic separation utilizing Waters ACQUITY UPLC BEH Technology enabled all isobaric steroid hormones in the panel to be resolved in under 10 minutes, allowing for selective quantification at low levels using the Xevo TQ-XS Tandem Mass Spectrometer from only 200 μL of serum.

**For Research Use Only. Not for use in diagnostic procedures.**

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