An Automated Compliance-Ready LC-MS Workflow for Intact Mass Confirmation and Purity Analysis of Oligonucleotides

Library Number:
APNT135057716
Part Number:
720006820EN
Author(s):
Catalin Doneanu, Jonathan Fox, Emma Harry, Chris Knowles, Ying Qing Yu, Joseph Fredette, Weibin Chen
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 
 

This application note describes a streamlined, compliance-ready workflow for oligonucleotide analysis employing the BioAccord System operating under waters_connect HUB data acquisition and processing software.

  • A waters_connect workflow is shown to provide good mass accuracy (5–15 ppm) for intact mass confirmation of oligonucleotides of various lengths (10–35 mers), as well as phosphorothioated oligos. 
  • In addition, the automated processing method that was developed provides purity information for all the oligo sample components that are separated by ion-pair reversed-phase chromatography.
  • This automated, compliance-ready LC-MS workflow provides for a robust, easy-to-use solution for fast mass confirmation and purity analysis of oligonucleotides.

Title Format File Size
Download PDF PDF 4555.92kB