Anion-Exchange Chromatography for Determining Empty and Full Capsid Contents in Adeno-Associated Virus

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Hua Yang, Stephan Koza, Weibin Chen
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Application Notes
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Evaluating empty and full capsid content is important, because it can help to ensure the product quality as well as to help determine the appropriate dosage of the drug.

Here, we demonstrated that with proper method development, AAV8 empty and full capsids can be separated and their relative abundances determined using a Waters Protein-Pak Hi Res Q Column.

Due to differences in capsid protein surfaces, method evaluation, and perhaps some method optimization may be needed when separating empty and full capsids of other AAV serotypes, and the AEX method in this application note can serve as a useful starting point.

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