Impact of Instrument Characteristics on Reversed Phase Chromatography Method Transfer Across Biocompatible UHPLC Systems

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Zhimin Li, Paula Hong, and Patricia McConville
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In this study, we will demonstrate the transfer of an RP method across ACQUITY Arc Bio System and Agilent 1260 Infinity Bio-inert System. We will show retention time and peak area percent repeatability across these two LC systems. A column, specifically designed for separation of intact monoclonal antibody (mAb) and subunits, Waters™ BioResolve RP mAb Polyphenyl Column, will be used for the study. The chromatographic system characteristics, including system dispersion and dwell volume, will be evaluated on their impact on resulting chromatography. In the end, we will explain the features of the ACQUITY Arc Bio System, Multi-flow path Technology and Gradient SmartStart and how they enable the gradient delay of other systems to be emulated without the need for adjustments to the gradient table.

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