Determination of Isoflavones in Dietary Supplements: A Comparison of Mass Detection with UV Detection

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Jinchuan Yang, Mark Benvenuti, and Gareth Cleland
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Application Notes
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Application Notes
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In a previous application note, we discussed the benefits of the Waters ACQUITY QDa Mass Detector for method development and transfer of a USP isoflavones method using a Waters CORTECS C18 Column and the ACQUITY Arc UHPLC System.

Using this new method, the LC run time was reduced from 74 minutes to 18 minutes. The ACQUITY QDa Mass Detector was used in conjunction with a PDA detector to locate the target peaks and identify co-elution issues.

In this application note (Part 3 of 3), we focus on the quantitative aspect of mass detection. The concentrations of six isoflavones (daidzein, glycitein, genistein, daidzin, glycitin, and genistin) in dietary supplements were determined by the ACQUITY Arc UHPLC method with both PDA and mass detection.

This study demonstrates that in the analysis of complex samples, such as plant extracts, the use of mass detection greatly reduces the interferences from co-eluting compounds. Furthermore, time-consuming troubleshooting for out of specification results are reduced, which will in turn can improve overall lab productivity and analytical data quality.


Part 1: Fast Analysis of Isoflavones in Dietary Supplements – USP Method Transfer onto a UHPLC System

Part 2: Fast Analysis of Isoflavones in Dietary Supplements – Benefits of Mass Detection in Method Transfer and Sample Analysis

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