Developing a Novel, Integrated LC-MS Workflow for High-resolution Monitoring and Characterization of Oligonucleotides

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Henry Shion, Robert Birdsall, and Ying Qing Yu
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Application Notes
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This application note describes an integrated LC-MS workflow for identification, impurity profiling, and MS/MS characterization of oligonucleotides using high-resolution mass spectrometry.

The complete workflow consists of an ACQUITY UPLC H-Class Bio System coupled to an ACQUITY UPLC Tunable Ultraviolet (TUV) Detector and the Xevo G2-XS QTof Mass Spectrometer. System control and MS data acquisition is accomplished using Waters MassLynx Mass Spectrometry Software.

High-resolution oligonucleotide characterization was demonstrated by performing a full ladder sequencing of a siRNA sample using the MS/MS spectrum within MassLynx.

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