Antioxidant Isolation Using the Prep 150 LC System

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Catharine Layton and Andy Aubin
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Application Notes
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This application note demonstrates how the purity of chlorogenic acid which is isolated from Aronia berries can be maximized by focusing the gradient to improve resolution of the compound of interest.

Throughput was maximized by first generating a focused gradient, then optimizing the focused gradient for run time. The purification method effectively yielded a chlorogenic acid isolate comparable in purity to the reference standard at 324 nm. The entire purification when scaled from analytical to prep, was successfully accomplished using the Waters Prep 150 LC System.

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