Combining RapiFluor-MS and UNIFI Software for a Total N-linked Glycan Solution for Comparing Innovator vs. Biosimilar Infliximab

Library Number:
APNT134899853
Part Number:
720005753EN
Author(s):
William R. Alley, Jr. and Ying Qing Yu
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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In this application note, we describe a streamlined N-linked glycan analysis solution for comparing an innovator biologic with a biosimilar, infliximab.

Waters has developed a total glycan analysis solution – beginning with the sample preparation using the GlycoWorks RapiFluor-MS N-Glycan Kit, transitioning to automated data collection and processing, continuing to data interpretation, and concluding with report generation using the Glycan Application Solution with UNIFI. This total solution significantly increases throughput, streamlines the entire workflow, simplifies sample preparation, and enables a broader community to successfully engage in glycan analysis.

UNIFI Software allows many different types of investigations to be conducted, and this application note demonstrated a comparison of innovator vs. biosimilar mAbs. For this type of comparative study, the tools within UNIFI Software allow users to quickly identify which glycans are present at different abundance levels between the different sample types.

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