Using Hydrophilic Interaction Chromatography for Heightened Product Characterization to Overcome Challenges with Hydrophobic Monoclonal Antibodies and Antibody Drug Conjugates

Library Number:
APNT134888711
Part Number:
720005684EN
Author(s):
Jacquelynn Smith, Matthew A. Lauber, Stephan M. Koza, Erin E. Chambers, Jason C. Rouse, and Olga V. Friese
Source:
Waters, Pfizer Inc
Content Type:
Application Notes
Content Subtype:
Application Notes
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This application note describes chromatographic recovery of hydrophobic mAb and antibody drug conjugate (ADC) subunits.

HILIC has been found to be complementary to RPLC and a promising new tool for biotherapeutic protein characterization. While HILIC of mAb subunits can be used to assess N-glycosylation profiles, this study shows that it can also be taken advantage of to more comprehensively recover the subunits of mAbs and ADCs.


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