Using Hydrophilic Interaction Chromatography for Heightened Product Characterization to Overcome Challenges with Hydrophobic Monoclonal Antibodies and Antibody Drug Conjugates

Library Number:
Part Number:
Jacquelynn Smith, Matthew A. Lauber, Stephan M. Koza, Erin E. Chambers, Jason C. Rouse, and Olga V. Friese
Waters, Pfizer Inc
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:

This application note describes chromatographic recovery of hydrophobic mAb and antibody drug conjugate (ADC) subunits.

HILIC has been found to be complementary to RPLC and a promising new tool for biotherapeutic protein characterization. While HILIC of mAb subunits can be used to assess N-glycosylation profiles, this study shows that it can also be taken advantage of to more comprehensively recover the subunits of mAbs and ADCs.

Title Format File Size
Download PDF PDF 1456.4kB