Quantification of the Antibody Drug Conjugate, Trastuzumab Emtansine, and the Monocolonal Antibody, Trastuzumab in Plasma Using a Generic Kit-Based Approach

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Hua Yang, Mary Lame, Sherri Naughton, and Erin Chambers
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Application Notes
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Application Notes
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Protein Quantification

This application note describes the total mAb quantification of the antibody-drug conjugate, ado-trastuzumab emtansine, and the mAb, trastuzumab from rat plasma using the ProteinWorks eXpress Direct Digest Kit and Protocol.

The ProteinWorks eXpress Direct Digest Kit was successfully used for sample preparation prior to LC-MS quantification of trastuzumab and T-DM1 from a typical set of standard curve and QC samples in plasma. The universal, kit-based approach to sample prep allows novice users to achieve high LC-MS sensitivity with a simple step-wise protocol and standardized, premeasured reagents, ensuring both the analytical sensitivity and reproducibility required in discovery studies to make time sensitive and critical project decisions.

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