In this application note a method for diclazuril based of the USP monograph and scaled to a 1 mm I.D. column is used to demonstrate the extra column dispersion and the effect it has on the resulting chromatography.
In order to achieve high quality separations using 1.0 mm I.D. columns, the user must be cognizant of the LC instrument characteristics and the potential negative effects it may have on the resultant chromatography. In order to maximize resolution between peaks, it is important to have the lowest possible dispersion in the instrument. The ultra-low dispersion of the ACQUITY UPLC I-Class System provides exceptional chromatographic performance when running microbore columns.