Preparation and Reliability of an Empirical Table in Auto•Blend Plus for Reversed-Phase Separations

Library Number:
APNT134873625
Part Number:
720005568EN
Author(s):
Amanda B. Dlugasch, Thomas E. Wheat, and Patricia R. McConville
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Technical Notes
Related Products:

This technology brief demonstrates the predictability and robustness of an empirical table created for use in Auto•Blend Plus.

The defined protocol provides two buffer systems, one low pH range and one high pH range, for the specified components of formic acid and ammonia. The two buffer systems can be used for LC-MS analysis if the buffer solutions are prepared exactly as the protocol states. The protocol is reliable in controlling the variability associated with an empirical table and provides the ability to create an empirical table across multiple laboratories.


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