Quantitative Analysis of THC and Metabolites in Urine With a Simple, Fast, and Clean Oasis PRiME HLB μElution Plate

Library Number:
APNT134872518
Part Number:
720005556EN
Author(s):
Dr. Xin Zhang, Dr. Jonathan P. Danaceau, and Dr. Erin E. Chambers
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
Forensic Toxicology
 
 

This application note details the extraction and UPLC-MS/MS analysis of THC and its metabolites from urine using Oasis PRiME μElution Plates.

The use of Oasis PRiME resulted in consistent and highly reproducible recoveries of all compounds with minimal matrix effects. The μElution format allowed for the concentration of the sample on the SPE column, eliminating the need to evaporate and reconstitute the sample, minimizing the risk of analyte loss due to nonspecific binding and streamlining the laboratory workflow. This resulted in a method that was linear, accurate and precise for all analytes, with limits of quantification of 0.1 ng/mL for THC and its metabolites.


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