Quantitative Analysis of THC and Metabolites in Urine With a Simple, Fast, and Clean Oasis PRiME HLB μElution Plate

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Dr. Xin Zhang, Dr. Jonathan P. Danaceau, and Dr. Erin E. Chambers
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Application Notes
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Application Notes
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Forensic Toxicology

This application note details the extraction and UPLC-MS/MS analysis of THC and its metabolites from urine using Oasis PRiME μElution Plates.

The use of Oasis PRiME resulted in consistent and highly reproducible recoveries of all compounds with minimal matrix effects. The μElution format allowed for the concentration of the sample on the SPE column, eliminating the need to evaporate and reconstitute the sample, minimizing the risk of analyte loss due to nonspecific binding and streamlining the laboratory workflow. This resulted in a method that was linear, accurate and precise for all analytes, with limits of quantification of 0.1 ng/mL for THC and its metabolites.

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