Charge Reduction Electron Transfer Dissociation (crETD) and Ion Mobility for Deconvolution of Protein Mass Spectra and Conformational Analysis

Library Number:
APNT134869573
Part Number:
720005484EN
Author(s):
Frederik Lermyte, Jonathan P. Williams, Jeffery M. Brown, Esther M. Martin, Frank Sobott
Source:
Waters, University of Antwerp
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
SYNAPT G2 MS
 
SYNAPT G2 MS
 

This application note describes a method of extensive charge reduction of native protein complexes on unmodified SYNAPT G2 and SYNAPT G2-S Ion Mobility Mass Spectrometers.

This method was used to partially deconvolute the very complex signal generated by a large, polydisperse assembly, allowing identification of the major species present. Concomitant top-down fragmentation provides sequence information. In combination with ion mobility, the effect of charge state on the global conformation of proteins and protein complexes can be studied.


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