This application note demonstrates that an established ion-exchange method for the separation of a monoclonal antibody can be easily replicated and can yield repeatable results.
The ACQUITY Arc System offers users the ability to emulate legacy HPLC methods or update methods to UHPLC separations through the use of Arc Multi-flow path technology. This application note demonstrated the ease of transferring an IEX method from an Agilent 1100 Series HPLC System to the ACQUITY Arc System. When keeping all method parameters the same, resulting chromatograms had relative retention times that were virtually indistinguishable from one another. Further inspection of five replicate injections on the ACQUITY Arc System shows a high degree of reproducibility.