Utilising Mixed Mode μelution SPE for the LC-MS/MS Analysis of Steroid Hormones in Serum for Clinical Research

Library Number:
PSTR134865688
Author(s):
Dominic Foley, Brian Keevil, Lisa J Calton
Source:
Waters, University Hospital of South Manchester
Content Type:
Posters
Content Subtype:
Other Symposium
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Here we evaluate an LC-MS/MS method used to measure serum tes tos terone and androstenedione enabling investigation of metabolic dysfunction for clinical research purposes. An analytically selective method was developed using a mixed-mode Solid Phase Extraction (SPE) sorbent in 96-well plate μElution format. The use of this μElution chemistry provides precise and accurate measurement of testosterone and androstenedione to high analytical sensitivity.


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