Supercritical fluid chromatography (SFC) has become a mainstream technology for chiral separations based on its higher efficiency, throughput, and wide applicability. For purification purposes, chiral SFC has seen increased interest and applicability, becoming the technique of choice in many cases. Purification of chiral compounds by SFC typically utilizes isocratic method conditions and stacked injections for optimal collection efficiency. In this application note, bucetin will be used as an example compound to demonstrate the typical work flow for chiral purification by SFC. The process will include gradient screening and isocratic method development on the ACQUITY UPC2 System, scale-up and purification using stacked injections on the Prep 80q SFC System, and post purification analysis on the ACQUITY UPC2 System.