In this application note we present the use of RapiFluor-MS, a novel reagent for rapidly labeling released N-glycans.
For routine high throughput assays, RapiFluor-MS with the ACQUITY UPLC H-Class Bio System and the ACQUITY QDa Detector provides a novel approach for accurately monitoring released N-glycan species. Reduced sample preparation times and greatly improved MS response when coupled with the ACQUITY UPLC and ACQUITY QDa enable the ability to more closely monitor released N-glycan profiles, something which has previously not been possible. While FLR detection was used in this example, for high throughput methods requiring only relative quantification this may not be needed as each species can be monitored with the ACQUITY QDa Detector. As discussed here, complete sample preparation and analysis can be completed in 40 minutes. In addition to reproducible sample preparation, separation and quantification are reproducible and quantitative, allowing scientists to make meaningful decisions rapidly.