In this study, the Forensic Toxicology Application Screening Solution with UNIFI was applied to a selection of plant alkaloids. The ease by which the scientific library items can be created and updated has been clearly demonstrated. The UNIFI Scientific Information System v1.8 was used to process the MSE data and for these plant alkaloids multiple adducts were detected. The fragment match functionality was also able to assign sub-structures to high-energy ions. Additionally, the new discovery tool has been shown to enhance the elucidation of unknown components.