Pesticide residue analysis in food has become a more challenging task considering the increasing number of compounds and complex food commodities to be monitored at low concentrations with generic extraction procedures. The direct consequences are complex extracts (presence of matrix compounds), for which multiple injections have to be performed while achieving dwell time and duty cycle balance. Screening methods are a practical alternative and full scan high resolution MS (HRMS) offers high specificity and the ability to detect a large number of analytes simultaneously using generic instrumentation parameters. Advances in mass spectrometry have vastly improved sensitivity for full spectral analysis, but further sensitivity enhancements would improve the mass spectral data quality. This is especially important to avoid compromised precursor ion or fragment ion information, and ensure high mass accuracy below the legislated levels. In this application note we explore the improved selectivity of ion mobility collision cross sections (CCS) measurements in combination with iKey microflow-LC technology for screening pesticide residues in food.