The genus Passiflora consists of approximately 450 species, a few of which are commercially exploited in functional food products such as teas and juices. These species contain flavonoids, one of the largest and most widespread classes of compounds which possess diverse pharmacological/biological properties.
Legislative focus has resulted in the expansion of method development to support the analysis of active compounds in and authenticity of functional food products and dietary supplements. For example, the European Union’s Directive 2004/24/EC came into full effect on 30 April 2011. Hundreds of traditional herbal remedies were banned, as the EU directive aims to protect consumers from possible damaging side-effects of over-the-counter herbal medicines.
Recent regulations allow only long-established and quality-controlled products to be sold. Manufacturers have to prove that their products are been made to strict standards and contain a consistent and clearly marked dose.
ionKey/MS ion mobility mass spectrometry (IM-MS) can provide a route to specific and unambiguous identification at low detection levels.
In this application note, a novel microflow technique using the ACQUITY UPLC M-Class System and IM-MS that leverages both positive and negative ionization has been developed to analyze the extracts of Passiflora edulis, alata, caerulea, and incarnata.
For the first time, chromatographically coeluting isobaric flavonoids have been separated using ion mobility mass spectrometry, enabling the individual calibration curves for isovitexin and vitexin to be obtained.