The development of analytical methods for the separation of chiral compounds is important in many areas of research, as it is well known that different enantiomers are selectively biologically active. Biochemical reactions can be diastereo or enantioselective. While one isomer may deliver the desired effect to the target species, the other enantiomer may be less effective to the target, completely ineffective, or cause undesirable effects. Additionally, it is known that different isomers can have very different environmental fates. It is estimated that 20 to 30% of pesticides on the market today have optical isomers, and there are reports that 40% of the pesticides used in China are chiral. The study of enantioselectivity is important to the crop protection industry, since the knowledge of the efficacy of each individual enantiomer could facilitate a significant reduction in the total amount of pesticide applied. In order to improve our knowledge of the stereoisomeric compositions of these substances, analytical methods that provide reliable and reproducible separations in a rapid time frame are necessary. Supercritical fluid chromatography (SFC) is known as an effective chiral separations technique that has many advantages over conventional high performance liquid chromatography (HPLC). In this application note we present the enantiomeric and/or diastereomeric resolutions of 12 triazole fungicides using Trefoil Column Technology. Trefoil Columns use a modified polysaccharide chiral stationary phase (CSP) with a 2.5 μm particle designed for broad-spectrum chiral selectivity. Resolutions were performed using an UltraPerformance Convergence Chromatography (UPC2) System. Convergence chromatography is a complimentary separation technique to liquid chromatography, that provides orthogonal selectivity, and uses supercritical CO2 as the primary mobile phase.