Transferring RapiFluor-MS Labeled N-Glycan HILIC Separations Between UPLC and HPLC

Library Number:
APNT134839414
Part Number:
720005344en
Author(s):
Stephan M Koza, Matthew A Lauber, and Kenneth J Fountain
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
Alliance HPLC Bioseparations
 
Alliance HPLC Bioseparations
 
 
 
 

In the following work, we demonstrate that Glycan BEH Amide Columns packed with 1.7 μm and 2.5 μm particle sizes afford scalability between RapiFluor-MS labeled glycan separations performed under UPLC and HPLC-compatible conditions. Using standard LC method transfer principles to account for differences in particle diameter (dp), column length, and column internal diameter, we show that comparable chromatographic profiles and relative quantitation can be achieved with the larger particle size column at HPLC-compatible pressures, albeit with an increase in sample load, mobile phase use, and most importantly, analysis time.


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