Generation of Multiple Images from a Single Tissue Section with Dual Polarity Desorption Electrospray Ionization Mass Spectrometry
Here we demonstrate that by optimizing DESI imaging conditions, a wealth of molecular information can be accessed from a single tissue section. Multiple images containing unique information can be collected from a tissue sample without the need for the analysis of a number of serial sections.
The advantages of DESI imaging include:
- Optimization of DESI conditions allows multiple imaging experiments to be performed on the same tissue sample without altering the architecture or composition of the tissue
- Both positive and negative ion mode DESI imaging data can be collected from a single tissue section
- Using optimized DESI conditions enables subsequent analysis of tissue sections by other visualization techniques (i.e. H&E staining)