Purification of Cannabidiol from Hemp Oil Using the Prep150 LC System

Library Number:
APNT134830443
Part Number:
720005287EN
Author(s):
Andrew Aubin
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 

This application note will describe the purification of Cannabidiol CBD from a CBD-rich paste using the Waters® Prep 150 LC System.

A simple purification workflow, starting with a high-speed assay method using UPLC, followed by analytical scale HPLC method development, scale-up to prep, and finally preparative scale injections and collections were shown. Starting from a concentrated hemp paste, five preparative scale injections produced 15 mg of high purity CDB. Chromscope Software allowed for easy collection set-up, along with simple operation of the Prep 150 LC System. The ACQUITY UPLC H-Class System provided the flexibility to run high-speed (<3 minutes) UPLC assays, as well as analytical HPLC method development, which was scalable to the Prep 150 LC System.


Title Format File Size
Download (720005287en) PDF 1844.3kB