This application note will describe the purification of Cannabidiol CBD from a CBD-rich paste using the Waters® Prep 150 LC System.
A simple purification workflow, starting with a high-speed assay method using UPLC, followed by analytical scale HPLC method development, scale-up to prep, and finally preparative scale injections and collections were shown. Starting from a concentrated hemp paste, five preparative scale injections produced 15 mg of high purity CDB. Chromscope Software allowed for easy collection set-up, along with simple operation of the Prep 150 LC System. The ACQUITY UPLC H-Class System provided the flexibility to run high-speed (<3 minutes) UPLC assays, as well as analytical HPLC method development, which was scalable to the Prep 150 LC System.