Analysis of Deamidation and Oxidation in Monoclonal Antibody using Peptide Mapping with UPLC/MSE

Library Number:
APNT10099045
Part Number:
720002897en
Author(s):
Hongwei Xie, Martin Gilar, and John C. Gebler
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 
SYNAPT MS

This application note demonstrates that UPLC/MSE is a suitable tool for characterizing PTMs in monoclonal antibodies. Using UPLC/MSE with the ACQUITY UPLC and SYNAPT MS systems, the identification of peptide sequences and determination of site-specific moditications can be accomplished in a single LC run because of available peptide fragmentation information provided by MSE.


Title Format File Size
Download 720002897en PDF 1932.5kB