Utilization of the Arc Premier System and XSelect Premier Columns for Sensitive LC-MS Quantitation of the Steroid Phosphates, Betamethasone, Dexamethasone, and Hydrocortisone

Library Number:
APNT135084971
Part Number:
720007269EN
Author(s):
Mary Trudeau, Fadi L. Alkhateeb, Paul D. Rainville
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Technical Notes
Related Products:
 
 
Pharmaceutical Discovery
 
 
 
 

The use of the Arc Premier System and XSelect Premier HSS T3 Column enabled the development of a sensitive quantitative MRM method for steroid pharmaceutics, achieving LLOQs of 1 ng/mL. The Arc Premier System and XSelect Premier Columns with MaxPeak HPS Technology greatly improved recovery of the steroid-phosphates, betamethasone, dexamethasone, and hydrocortisone, while demonstrating equivalent performance for the non-phosphate steroids, dexamethasone, and dexamethasone acetate. This proof of concept method shows great promise for accurate quantification of steroids in support of drug discovery, research, and manufacturing.


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