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Best Practices for Oligonucleotide Analysis Using Ion-Pair Reversed-Phase (IP-RP) Liquid Chromatography – Columns and Chemistries

Library Number:
APNT135071755
Part Number:
720006948EN
Author(s):
Martin Gilar, Nicholas J. Zampa
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
Oligonucleotides Separation Technology Columns
 
ACQUITY UPLC System
 
ACQUITY UPLC PDA Detector
 
Alliance HPLC
 
Oligonucleotide Analysis
Abstract / Summary

In support of customers working on oligonucleotides, Waters offers these critical best practices to ensure consistent high-performance IP-RP liquid chromatographic separations for oligonucleotide analysis. Robust methods are critical to delivering high-quality oligonucleotides in diagnostic or therapeutic applications. For example, high-quality oligonucleotide primers and probes are essential for accurate PCR-based diagnostic assays, including those for COVID-19.


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