The European Union Reference Laboratory for Pesticides Single Residue Methods (EURL-SRM) published the QuPPe (Quick Polar Pesticides) methods for the simultaneous analysis of a number of highly polar pesticides. To meet the needs of analyzing highly polar pesticides by LC-MS/MS, details on a number of chromatographic methods have been provided including one based on hydrophilic interaction liquid chromatography (HILIC) for the determination of a series of cationic and polar basic analytes.
In this application note, example performance data is provided from ACQUITY UPLC I-Class System and Xevo TQ-S micro on three commodities which represent high water content and high starch, low water content sample types. Organic wheat flour, cucumber, and apple, were extracted following the QuPPe method, to assess various performance factors of the UPLC-MS/MS method such as calibration linearity, retention time stability, method precision, and trueness.
Overall the performance data indicate that the configuration of the ACQUITY UPLC I-Class coupled with Xevo TQ-S micro, when used in combination with the ACQUITY UPLC BEH Amide Column and an established extraction protocol such as QuPPe, is suitable for checking MRL/tolerance compliance in routine laboratory testing for these target compounds.