Determination of Anionic Polar Pesticides in Wheat Flour Extracts Using UPLC-MS/MS with the Torus DEA Column

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Dimple Shah, Gareth Cleland, Euan Ross, Eimear McCall, Benjamin Wuyts, and Simon Hird
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Application Notes
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Application Notes
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ACQUITY UPLC H-Class Bio System

Although various multi-residue LC-MS/MS methods are available to analyze food for pesticide residues, polar, anionic pesticides and their metabolites remain a considerable challenge. The QuPPe (Quick Polar Pesticides) method allows the simultaneous extraction of many of these highly polar compounds. QuPPe is typically used with LC-MS/MS instruments offering high sensitivity in order to deal with the significant matrix effects associated with the crude extracts (no cleanup).

In this application note, we report the performance data from an assessment of the slightly modified LC-MS/MS method for the determination of relevant anionic polar pesticides in wheat flour extracts, representative of commodities with high starch and/or protein content, low water and fat content.

This method is suitable for the determination of a range of polar anionic pesticides in wheat flour extract to facilitate monitoring of MRL/tolerance compliance. The method offers excellent chromatographic retention, selectivity, peak shape, and stability coupled with sufficient sensitivity to determine residues at concentrations as low as 0.01 mg/kg (10 ppb) levels in crude extracts without cleanup.


*Please note: This Application Note was developed on a Torus DEA Column, improved performance can be achieved using the Waters Anionic Polar Pesticide Column coupled with our most recent Application Notes please contact Waters Chemistry Technical Services with any questions

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