Utilization of the Arc Premier System and XSelect Premier Columns for Sensitive LC-MS Quantitation of the Steroid Phosphates, Betamethasone, Dexamethasone, and Hydrocortisone

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Mary Trudeau, Fadi L. Alkhateeb, Paul D. Rainville
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Application Notes
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Technical Notes
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Pharmaceutical Discovery

The use of the Arc Premier System and XSelect Premier HSS T3 Column enabled the development of a sensitive quantitative MRM method for steroid pharmaceutics, achieving LLOQs of 1 ng/mL. The Arc Premier System and XSelect Premier Columns with MaxPeak HPS Technology greatly improved recovery of the steroid-phosphates, betamethasone, dexamethasone, and hydrocortisone, while demonstrating equivalent performance for the non-phosphate steroids, dexamethasone, and dexamethasone acetate. This proof of concept method shows great promise for accurate quantification of steroids in support of drug discovery, research, and manufacturing.

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