Development of a Sensitive High Resolution Mass Spectrometry Method for Quantitation of N Nitrosamines using Tof MRM on the Xevo G2-XS QTof

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Lauren Mullin Margaret Maziarz Mary E. Lame Nathan Anderson
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Methods for the analysis of potentially carcinogenic N-nitrosamines require sufficient sensitivity to accurately measure at sub-ng/mL concentrations. Here we have demonstrated that using Tof MRM on the Xevo G2-XS QTof MS coupled with UPLC chromatographic separation, LLOQ levels at or below 0.5 ng/mL for NDMA, NDEA, NMBA, NDIPA, NEIPA, and NDBA were achieved. Quantitative testing of the method also demonstrated reliable and accurate measurement (within 15%) of two QC levels in the low ng/mL range for NDMA and NDEA. Overall, the ACQUITY UPLC coupled to Xevo G2-XS QTof MS with the UNIFI Scientific Information System offers a modern platform for GxP-compliant, sensitive, selective, and specific HRMS quantitation of N-nitrosamines.

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